Fig. 3. Nuclear Clik1 is relocalized when coexpressed with CLP-36. U2OS osteosarcoma cells were transfected with the following expression vectors: Myc-Clik1 (A-C), HA-CLP-36 (D-F), Myc-Clik1 and HA-CLP-36 (G-I), Myc-CDK7 and HA-CLP-36 (J-L). The subcellular localization of Clik1, CDK7 and CLP-36 was analyzed by double-label immunofluorescence with a mouse monoclonal anti-Myc (A,D,G,J) and a rabbit polyclonal CLP-36 antibody (B,E,H,K). Hoechst staining was used to visualize nuclei (C,F,I,L). Bars, 15 µm.