Fig. 5. The C-terminal LIM domain of CLP-36 is essential for Clik1 relocalization. U2OS cells expressing EGFP-CLP-36 and myc-Clik1 (A,B,I,J), EGFP-CLP-36 and the kinase-deficient myc-ClikK98M (C,D), a C-terminal mutant lacking the LIM domain EGFP-CLP1-231 and myc-Clik1 (E,F) or EGFP-CLP-36 alone (G,H) were immunostained with mouse monoclonal anti-myc to detect Clik1 (B,F) and ClikK98M (D) or were immunostained with Texas-Red-conjugated phalloidin (H,J) to demonstrate actin filaments. Bars, 15 µm. Anti-CLP-36 (upper panel) and anti-Myc (lower panel) western blot analyses were used to control the expression of fusion proteins used for immunofluorescence studies (K).