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Fig. 6. Both Clik1 and CLP-36 are expressed in adult testis. (A) Human multiple-tissue northern blot filters containing the indicated mRNAs (Clontech, no. 7760-1, 7766-1 and 7757-1) were probed with Clik1. The positions of the 7.5 kb mRNA standards are indicated on the right. (B) Western blot analysis from adult mouse testis extract with rabbit polyclonal CLP-36 antibody: total testis extract (lysate), anti-CLP-36 immunoprecipitate from testis extract (IP, {alpha}-CLP-36) and blocked anti-CLP-36 immunoprecipitate (IP, block). (C) Frozen adult mouse testis sections were fixed with ethanol and stained with rabbit polyclonal anti-CLP-36 or with blocked anti-CLP-36 (inset). The white arrow indicates the peritubular contractile cells surrounding the seminiferous tubuli, and the white arrowhead indicates the elongating spermatids. Bar, 40 µm.