Fig. 1. Activities of tie-1 and VE-cadherin promoters in cultured cells. 600 ng of each luciferase reporter construct were transfected into BAE (bovine aortic endothelial cell), CCL39 and 293 cells (non-endothelial cells). In each assay, 300 ng of the ß-galactosidase expression vector were co-transfected in order to normalize for transfection efficiency. 48 hours after transfection, luciferase activity was measured. The relative activity of each construct was expressed as fold induction over the pGL2 basic vector. The results shown in this figure are representative of three independent experiments. Each set of data represents the mean of quadruplicate determinations.