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Fig. 1. RNA binding and subcellular distribution of GFP-IMP1. (A) UV crosslinking of IGF-II leader 3 segment C RNA with extract from either NIH 3T3 cells that stably express GFP-IMP1 (lane 1) or from normal NIH 3T3 cells (lane 2). (B) Cytoplasmic lysate from GFP-IMP1-expressing NIH 3T3 cells was separated in a 20-47% sucrose gradient. The upper panel shows the A260 sedimentation profile of ribosomal subunits and polyribosomes, and the lower panel shows the western analysis of the 12 fractions from the Mg2+-containing gradient with anti-IMP1 antibody to IMP1 and GFP-IMP1. M is a lane with recombinant IMP1.