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Fig. 2. Cytoplasmic localization of GFP-IMP1 during interphase and anaphase in NIH 3T3 cells. (A) Cytoplasmic localization of GFP-IMP1 during interphase. (1) A cell with a delocalised granular pattern of fluorescence that is typically seen in cells seeded on glass plates. (2) A migrating cell with GFP-IMP1 localized to the leading edge (arrow). (3,4) The GFP-IMP1-containing granules. (B) Time-lapse microscopy of GFP-IMP1 during mitosis displayed in glow scale.