Fig. 7. Subcytoplasmic localization of IMP1 is mediated by the four KH domains. NIH 3T3 cells were transiently transfected with GFP-IMP1 deletion constructs. (A) Schematic representation of the constructs and their ability (+) to form granules and localise subcytoplasmically. ^*The KH2-4 construct exhibited a tendency to form immobile aggregates that could be mistaken for granules. (B) Representative pictures of the constructs. (C) Cotransfection of CFP-IMP1 and YFP-KH1-4. The frames are in pseudo-colours to aid visualisation: CFP fluorescence is shown in green, YFP fluorescence is shown in red, and regions of overlap are shown in yellow.