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Fig. 3. (A) Protein extracted from non-transfected and stably-transfected N/N1003A cells was immunoblotted with antibody to Cdk5 or p35. Endogenous Cdk5 is expressed equally well in non-transfected N/N1003A cells (1), Cdk5-transfected N/N1003A cells (2) and Cdk5T33-tranfected N/N1003A cells (3). The arrow points to exogenous his-tagged Cdk5. (B) Expression of p35 mRNA. RT/PCR was performed on RNA extracted from rat lens (lane 3) or N/N1003A cells (lane 4). Negative controls lacking reverse transcriptase were performed with each sample: rat lens (lane 2); N/N1003A cells (lane 5). Molecular weight markers are shown in lane 1.