Fig. 5. Localization of actin and vinculin. Cells were centrifuged (35 g) onto fibronectin-coated slides, and incubated as follows: (A,D) 5 minutes, 4°C; (B,E) 5 minutes, 37°C; (C,F) 2 hours, 37°C. Following incubation, cells were stained with phalloidin to detect F-actin (A-C) or with anti-vinculin antibody for immunocytochemical localization of vinculin (D-F).