Fig. 6. Cell adhesion as measured with a centrifugation assay. Cells were centrifuged (35 g) on to fibronectin-coated 96-well polyvinyl chloride (PVC) plates and incubated on ice for 5 minutes, at 37°C for 5 minutes or for 2 hours. The plates were then inverted and centrifuged at the indicated speed for 5 minutes to remove weakly bound cells. The remaining attached cells were stained with crystal violet and total adhesion was quantified by measuring absorbance at 540 nm. Open bars, nontransfected N/N1003A cells; gray bars, Cdk5-transfected cells; and hatched bars, Cdk5-T33-transfected cells. Results shown are the average of eight measurements ±s.e.