Fig. 8. The effect of Cdk5 overexpression on cell aggregation. (A) Dissociated cultured cells were incubated in the presence or absence of calcium, and the extent of cell aggregation was determined using a coulter counter threshold diameter of 8 µm. The total number of particles in the absence of calcium was divided by the number of particles formed in the presence of calcium, to calculate cell number/aggregate. (B) Immunoblot with N-cadherin antibody of the detergent insoluble fraction of N/N1003 cells incubated in the absence (lane 1) or presence (lane 2) of calcium. (C) Immunoblot with N-cadherin antibody of the detergent insoluble fraction of N/N1003 cells, Cdk5-transfected N/N1003 cells, and Cdk5T33-transfected cells incubated in the presence of calcium (top panel). The immunoblot was stained with Ponceau Red to confirm that equivalent amounts of protein were loaded. The major stained band is shown for comparison (bottom panel).