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Fig. 9. Effect of Cdk5 overexpression on formation of Cdk5-p35 complexes and p35 autophosphorylation. Cdk5 was immunoprecipitated from cell lysates and the immunoprecipitated proteins were immunoblotted with anti-p35 antibody. (1) N/N1003A cells; (2) N/N1003A cells stably transfected with Cdk5; (3) N/N1003A cells stably transfected with Cdk5T33.