Fig. 1. Flow cytometry of fresh CD34⁺- immunodepleted cells (A) and cells following 6 weeks of selection in vitro (B). (A) Immunomagnetic depletion of the initial mononuclear fraction resulted in approximately 100% elimination of CD34⁺ cells, whereas up to 80% of cells expressed CD45 antigen. (B) Following 6 weeks of culture of CD34⁺- depleted cells in conditions described in the Materials and Methods, CD45⁺ cells were reduced to almost negligible levels (less than 4% of the whole cell population).