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Fig. 6. Fertilization of GV-stage oocytes does not trigger the increase in PtdIns(4,5)P2. (A) Confocal images of PH-GFP labeling in a GV-stage oocyte. DNA is stained with Hoechst 33342. The corresponding bright field image is displayed on the left. (B) Changes in [Ca2+]i and PH-GFP distribution at fertilization of a GV stage oocyte. Similar results were obtained in 4 oocytes. For abbreviations, see Fig. 2 legend.