Fig. 7. Micromolar wortmannin inhibits the PtdIns(4,5)P₂ increase but not exocytosis. (A) Fertilization-induced changes in [Ca²⁺]_i and PH-GFP distribution recorded in an egg pre-treated with 30 µM wortmannin for 15 minutes. Similar results were obtained in eight eggs. For abbreviations, see Fig. 2 legend. (B) FITC-LCA labeling on (i) unfertilized MII oocyte; (ii) control fertilized egg; and (iii) wortmannin (30 µM, 15 minutes)-treated fertilized egg. See Fig. 5D legend for details on FITC-LCA labeling. Similar patterns of staining were observed in at least 12 cells, for each condition.