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Fig. 4. PKC isozyme localization in myoblasts plated on FN. Myoblasts were plated on FN for various times, methanol/acetone fixed and stained for individual PKC isozymes. This figure shows characteristic patterns of localization of {alpha}, {delta} and {epsilon}PKC as the cells attach and spread. These results were observed in more than 90% of the cells. {alpha}PKC is localized at focal adhesion sites (arrow) after 15 minutes on FN. After 15 minutes on FN, {delta}PKC revealed a Golgi-like staining (arrow), whereas after 1 hour, {delta}PKC showed a punctate staining pattern at the cell periphery (arrow). After 15 minutes, {epsilon}PKC was detected in the nucleus and in perinuclear regions (arrow), and became localized diffusely in the cytosol as well as in the nucleus after 1 hour on FN.