Fig. 7. Actin reorganization in 5-expressing cells plated on FN. Actin localization and stress-fiber formation were observed in 5-expressing cells plated on FN. Paraformaldehyde-fixed cells were stained for actin and stress fibers (F-actin) as indicated in the Materials and Methods. At early time points (15 minutes), actin was found at focal adhesion sites (arrow). With time, focal contacts were distributed uniformly across the cell surface, and fine stress fibers were found around the nucleus and at the periphery of the cell.