Fig. 3. Endosomal localization of heparanase. Human foreskin fibroblasts were incubated with 10 µg/ml recombinant 65 kDa heparanase, and labeled for endosomes (TRITC-labeled ConA) and lysosomes (LysoTracker) for 120 minutes. Following fixation and permeabilization, the cells were stained by immunofluorescence with anti-heparanase mAb 130. Note the colocalization of the endosomal staining (red) and the heparanase-containing granules (green). The overlap is represented in yellow when the images are superimposed (third panel from top) and in fluorescent ratio image analysis (bottom panel).