Fig. 4. Heparanase-containing granules do not contain the EEA1 early endosomal protein. Human foreskin fibroblasts were incubated with 10 µg/ml 65 kDa heparanase for 120 minutes. The cells were then fixed, permeabilized and subjected to double immunofluorescent staining of heparanase (green, left image) and EEA1 (red, middle image). The superimposed image (right image) shows no colocalization of heparanase and EEA1. Bar, 20 µm.