Fig. 2. Hematopoietic cells in the granulation tissue. Giemsa stained cryosections
of 10-day-old wounds (A,B) and 14-day-old wounds (C,D). At day 10, the number
of giemsa stained cells is similar in both transgenic (A) and non-transgenic
(B) animals, whereas at day 14, the number of giemsa stained cells is clearly
reduced in transgenic animals (C) in comparison with non-transgenic animals
(D). Arrowheads indicate newly formed epidermis; arrows indicate hematopoietic
cells. Bars, 50 µm. (E) Quantification of hematopoietic cells. Counting of
giemsa stained cells in 64 mm2 fields of a photograph from wounds
of at least four different animals revealed a significantly reduced number in
transgenic animals compared with non-transgenic animals at day 14 after
wounding (P<0.02). Error bars represent the standard
deviation.