Fig. 3. Characterization of hematopoietic cells in the granulation tissue. Immunohistochemistry using Mac1 (CD11b)-antibody for detection of macrophages (A,B), and naphthol-ASD-chloroacetate-esterase histochemistry for detection of neutrophils and mast cells (C,D). Transgenic mice (A,C) exhibited significantly fewer numbers of macrophages (A), as well as neutrophils and mast cells (C) in cryosections of 14-day-old wounds when compared with the non-transgenic controls (B,D, respectively). Arrows indicate macrophages (A,B), and mast cells or neutrophils (C,D); arrowheads indicate the epidermal/dermal junction (A-D). Bars, 50 µm.