Fig. 5. Apoptosis in the epidermis of 13-day-old wounds. (A,C) Apoptotic cells in the newly formed epidermis, which is stained by an antikeratin 14 antibody (red), were marked by TUNEL-labeling (green; arrow heads). (B,D) All cell nuclei were stained with DAPI (blue). In non-transgenic animals (A,B), a significantly higher number of apoptotic cells were detected compared with transgenic animals (C,D). (E) The percentage of apoptotic cells. Only 0.42±0.16% basal epidermal cells in transgenic animals undergo apoptosis, whereas in wild-type animals 0.77±0.10% basal apoptotic cells are found. Non-transgenic animals, n=5; transgenic animals, n=4; ^*P<0.005. Bars, 50 µm.