Fig. 2. Amount of PDGF-r upon agonist stimulation that is able to be restimulated. 1x10⁶ NIH3T3 cells were serum starved for 24 hours and then stimulated with 30 ng/ml PDGF-BB. PDGF-r was immunoprecipitated from lysates. (A) Anti-PDGF-r immunoblot. (B) Cells were stimulated with PDGF-BB during two time courses for 10 minutes, 45 minutes, 2 hours and 3 hours for the first set, and for 3 hours, before being washed and restimulated again for 10 minutes, 45 minutes, 2 hours and 3 hours for the second set. The antiphosphotyrosine immunoblot and the anti-PDGF-r normalisation are shown.