Fig. 1. The influence of PLC-1 on cell survival. (A) Null and Null+ cells were plated on either regular tissue culture plastic dishes to adhere or on tissue culture dishes coated with poly-HEMA for suspension culture. 10% FBS was added as indicated (+ Serum) and cell viability was measured 20 hours later by calculating the ratio of trypan-blue-positive cells to live cells. The open and closed bars represent Null and Null+ cells, respectively. Each bar indicates the average of triplicate assays. SF indicates serum-free media. (B) Null and Null+ cells were seeded on poly-HEMA coated dishes. 10% FBS or a broad spectrum caspase inhibitor Z-VAD-FMK (20 µM) were added as indicated. Cells were collected 20 hours later, washed with PBS and cell lysates were prepared using cell lysis buffer. Caspase 3 activity present in the cell lysates was determined as described in the Materials and Methods. Open and closed bars indicate Null and Null+ cells, respectively.