Fig. 4. Localization of LET-502 with myosin (NMY-2) in wild-type embryos, and localization of NMY-2 and actin in let-502 and mel-11 mutant embryos using digital deconvolution microscopy. Arrows indicate the ingressing or newly completed cleavage furrows. (A-C) LET-502 and NMY-2 colocalize at the ingressing furrow during cleavage. (D-I) NMY-2 and actin still accumulate and colocalize to the putative site of furrow formation in all of the let-502(sb106) and let-502(sb106RNAi) embryos examined. Note that NMY-2 also localizes to the spindle during metaphase (G). NMY-2 and actin (J-L) and LET-502 and NMY-2 (M-O) retain their localization at the furrow in a mel-11(it26) mutant embryo during cleavage. (A-C) Bar, 5 µm. n=3 by deconvolution and n=13 by IF. (D-I) Bar, 7 µm. n=11 let-502(sb106RNAi) stained for both NMY-2 and actin by deconvolution and n=5 let-502(sb106RNAi) embryos stained for NMY-2 only by IF. (J-L) n=4 stained for both NMY-2 and actin by deconvolution, n=23 stained for NMY-2 only by IF and n=2 stained for actin only by IF. (M-O) n=2 by deconvolution and n=23 by IF.