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Fig. 5. Localization of active rMLC and LET-502 in embryos using digital deconvolution microscopy. All images shown are merged with rMLC posphoserine 19/18 in green, LET-502 in red and DAPI in blue, and arrows point to ingressing cleavage furrows. (A) rMLC phosphoserine 19/18 and LET-502 colocalize in wild-type embryos. (B-C) The intensity of rMLC posphoserine 19/18 decreases in let-502(sb106) embryos and increases in mel-11(it26) embryos in comparison with wild-type embryos. (D,E) rMLC phosphoserine 19/18 is not detectable or is present at low levels in mlc-4(RNAi) embryos. (F) rMLC phosphoserine 19/18 levels are restored in let-502(sb106RNAi); mel-11(it26) embryos. Bar, 6 µm. (A) n=4, (B) n=4, (C) n=2, (D) n=1, (E) n=1 and (F) n=6.