Fig. 6. LET-502 and MEL-11 require MLC-4 and early CYK-1 function for their localization but do not require late CYK-1 function or CYK-4. (A-C) LET-502 and MEL-11 no longer localize to the site of cleavage furrow formation in cyk-1(s2833/t1568) embryos, which show the early cyk-1 phenotype, or in mlc-4(RNAi) embryos (D-F). (G-I) LET-502 and MEL-11 retain their localization in cyk-4(t1689) embryos. (J-L) LET-502 and MEL-11 retain their localization in cyk-1(t1568) embryos, which display the late cyk-1 phenotype. cyk-1(t1611) embryos gave similar results (data not shown). Bar, 6 µm. (A-C) n=5 by IF, (D-F) n=10 by IF, (G-I) n=7 by deconvolution and n=6 by IF and (J-L) n=8 by deconvolution.