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Fig. 6. CENP-B and CENP-C localize to different centromere domains in interphase chromosomes. (A-F) Immunoelectron microscopy showing simultaneous colocalization of CENP-B (10 nm gold particles) and CENP-C (6 nm gold particles). Each micrograph shows the centromere of an unidentified chromosome from a single cell nucleus. (A'-F') The maximum perimeter occupied by the CENP-B gold particles is marked for illustrative purposes. CENP-C particles within this perimeter are marked in red, while those outside the perimeter are marked in green. The bar in panel F' represents 100 nm.