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Fig. 2. Preparation of recombinant proteins. (A) The recombinant protein units DelN(1) (lane 1), DelNo(1) (lane 2), NoC(1) (lane 3), CP123(1) (lane 4), E123(1) (lane 5), E3Ca(1) (lane 6), DelN(2) (lane 7), DelNo(2) (lane 8), NoC(2) (lane 9), E3Ca(2) (lane 10) and oCP123(2) (lanes 11 and 12) were resolved by SDS-PAGE under non-reducing (lane 11) or reducing conditions (all other lanes) and stained with Coomassie blue. The asterisk marks the migration position of DelN proteins. (B) Western blots of trimeric DelN(1) (lane 1) and DelN(2) (lane 3), or monomeric DelNo(1) (lane 2) and DelNo(2), (lane 4), resolved under non-reducing conditions, transferred to nitrocellulose and probed with an antibody to TSP-1 (lanes 1,2) or an antibody to TSP-2 (lanes 3, 4). Molecular weight markers are indicated in kDa.