Fig. 3. Cell attachment to TSP recombinant protein units. (A) 5x10⁴ C2C12 cells (white bars) or VSM cells (shaded bars) were seeded on microtitre plates coated with 50 nM intact TSP-1, FN or 1 µM of the TSP units, and cell attachment quantified after 1 hour of incubation at 37°C. Attachment to TSP-1 or the modules is expressed as percentage of cell attachment to FN. The bars show the mean±s.e.m. of three independent experiments. (B) VSM cells use multiple adhesion systems to attach to the DelNo(1) unit. Cells were incubated in the presence of 1 mM GRGDS peptide, antibodies to integrin ß1, ß3, 2 or 4 subunits (all at 5 µg/ml), 100 µg/ml heparin, 300 µg/ml chondroitin sulphate A or 1 mM of CD47-binding peptide, KRFYVVMWKQVTQK, either alone or in combination. Cell attachment was quantified after 1 hour of incubation and is expressed as the percentage of cell attachment relative to untreated controls. Bars show mean values ± s.e.m. from three experiments.