Fig. 5. FcRn colocalizes with an ER marker in the absence of ß₂m. FO-1 (a-c,g-i,m-o) or FO-1ß2m expressing Myc-hFcRn (panels d-f,j-l,p-r) were fixed, permeabilized and stained with monoclonal anti-EEA1 (a,d), anti-lamp1 (g,j) or anti-ribophorin II (m,p) antibodies to label endosomes, lysosomes or the ER, respectively, and polyclonal anti-Myc antibodies (b,e,h,k,n,q) to detect Myc-hFcRn. In c,f,i,l,o,r, the staining for endosomes, lysosomes or ER (green) was merged with that for Myc-hFcRn (red). The panels show representative data for one of two clones analyzed.