Fig. 6. FcRn remains EndoH sensitive in the absence of ß₂m. Lysates of FO-1 cells (lane 1), FO-1ß2m cells (lane 7), FO-1 cells expressing Myc-hFcRn (lanes 2-6) and FO-1ß2m cells expressing Myc-hFcRn (lanes 8-12) were either directly blotted (lanes 1,2,7,8) or first incubated with enzyme buffer alone (lanes 3,5,9,11) or with EndoH (lanes 4,10) or glycosidase F (lanes 6,12) for 3.5 hours at 37°C. Blots were probed with monoclonal anti-Myc (-Myc; top panel) or polyclonal anti-ß₂m (-ß2m; bottom panel) antibodies. The data is representative for three independent experiments, each carried out with two different cell clones.