Fig. 1. Western blots (A) showing the distributions of NCS-1-EYFP and endogenous NCS-1 in postnuclear supernatants (PNS), total membrane fractions (M) and cytosolic fractions (C) obtained from wild-type and stably transfected PC12 cells. Western blots (B,C) showing the distributions of NCS-1-EYFP, endogenous NCS-1, synaptophysin (Syn) and SgII in fractions collected after (B) velocity gradient centrifugation of PNSs and (C) equilibrium gradient centrifugation of fractions 4-8 from the velocity gradient. The blots also show the distribution of the Na/K ATPase. Quantitative data (D) showing the distributions of NCS-1-EYFP, endogenous NCS-1, synaptophysin and SgII in fractions collected from the equilibrium gradient. Data were obtained using the NCS-1-EYFP band in the right panel of (C) and the NCS-1, synaptophysin and SgII bands in the left panel of (C).