Fig. 7. Dual-color images showing that the distribution of vesicles containing (A) NCS-1-EYFP and (B) synaptophysin-EGFP changes appreciably in 20 seconds. After 20 seconds, overlap (yellow) is associated mostly with vesicles along neurites (arrows) and with cytosolic NCS-1-EYFP, which generates a diffuse yellow background in the cell in A. Bars, 10 µm.