Fig. 3. Expression of GFP reporter transgenes in third instar larvae. (A) UgGA larva shows broad GFP signal in most tissues implying ubiquitous transcription from the Ubi-p63E promoter. (B) In the UgGH larva, the GFP signal is weaker in most tissues compared with the UgGA larva. However, strong expression is observed in the CNS and salivary glands, and a very strong expression is seen in the male gonads. (C) In the UnGA larva, GFP signal is primarily in the CNS. However, a very low level of expression is also found in other tissues including the male gonads. (D) In the UnGH larva, GFP signal is similar to UnGA, but strong signal is seen also in the male gonads. Arrow, CNS; asterisk, salivary gland; arrowhead, male gonad.