Fig. 6. Localization of ectopic HuD and ELAV in the wing disc cells. Third instar larval wing disc cells ectopically expressing HuD or ELAV under the control of dpp-GAL4 driver were dissected and immunoprocessed with anti-HuD and anti-ELAV antibodies. Propidium iodide was used to visualize nuclei. Confocal images were collected for wing pouch cells. Immuno-signal in green (A,D); propidium iodide signal in red (B,E); merged images (C,F). ELAV shows both nuclear and cytoplasmic localization (A,B,C). In contrast, HuD shows mainly cytoplasmic localization (D,E,F). For details see Materials and Methods.