Fig. 5. Distribution of plant Spc98p, -tubulin and MTs in G2 tobacco BY-2 cells. Immunolocalization with (A) anti-Spc98p/Alexa 488 and (B) anti--tubulin/Alexa 568 in the same cell. In (A), a slightly oblique cut through the z-stack clearly shows the nucleus (n), vacuole (v) and surrounding cytoplasm. In (B), the projection of three confocal views shows the preprophase band in formation. Immunolocalization with (C) anti--tubulin/Alexa 488 and (D) anti--tubulin/Alexa 568 in the same cell. DIC image of the cell (D, inset). Both Spc98p and -tubulin accumulate densely at the nuclear surface. Spc98p is not present along MTs, whereas -tubulin is detected. Spc98p spots are also present inside the nucleus, suggesting import of the protein. Bar, 10 µm.