Fig. 6. GFP and Spc98p-GFP fusion protein in live tobacco BY-2 cells observed in confocal microscopy. (A) GFP, detected by fluorescence, is diffuse in the cytoplasm and the nucleus. (B) DIC microscopy of the same cell. The Spc98p-GFP fusion protein (C) is detected on the nuclear surface and as regularly spaced cortical signals close to the plasma membrane (arrows). (D) DIC microscopy of the same cell. (E,F) Plasmolysis recovery in DIC microscopy. (G-L) The same cell expressing Spc98p-GFP fusion protein. The fluorescent signals observed using time-lapse microscopy in the cortical area detailed in E, close to the plasma membrane shows slight displacements. During recovery (J-L), the fluorescent signals moved according to the plasma membrane movements, indicating a close relationship. (Large dotted line, cell wall; small dotted line, edge of the plasma membrane). (M) Movements of both GFP dots labelled by an arrow and an arrowhead from G to L are drawn. (N,O) Microtubule immunolabeling in a cell expressing Spc98p-GFP. The reconstructed cross-section shows that GFP fluorescence localizes at microtubule bundle ends. n, nucleus. v, vacuole. Bar, 10 µm.