Fig. 3. Effect of AICAR exposure on the abundance of cell-surface GLUT1. Clone 9 cells were exposed for 60 minutes to buffer only (a) or to 500 µM AICAR (b) or for 24 hours to 250 µM CoCl₂ (c). Plasma membrane lawns were prepared, immunostained and quantified as described in the Materials and Methods. Data were pooled from two experiments in which eight fields were measured, each containing an average of 12 cells. The image intensity data (arbitrary units) shown beneath the images are means±s.e.m. [(8) number of fields]. ^*Significantly different (P<0.05) from the control (ANOVA plus Bonferroni's ad hoc test); NS, not significantly different from the control. Scale bar, 100 µm.