Fig. 5. Comparison of the effects of exposure to azide and of infection by an adenovirus encoding ca-AMPK, or lacking AMPK, on hexose transport. Clone 9 cells in triplicate culture dishes were maintained uninfected (none) or infected with adenovirus either encoding ca-AMPK (ca) or lacking the AMPK gene (null) for 48 hours. The cells were washed twice with PBS and treated for 30 minutes with (azide) or without (basal) 5 mM azide. Uptake of [³H]3-O-methyl-D-glucose was then measured as described in the Materials and Methods. Results shown in (a) are means±s.e.m. (3). In parallel, western blots of cell lysates (10 µg protein) were prepared as described in the Materials and Methods section and stained with mouse antibodies to c-myc (b). The mobilities of molecular weight markers are indicated on the left. ^*This value is significantly different (P<0.05) from basal uptake rate in non-infected cells and from basal uptake rate in cells infected with adenovirus lacking the AMPK coding region.