Fig. 1. Subcellular distribution of GFP-Rab11 in K562 cells. K562 cells were transfected with the plasmid pEGFP (control) or with the constructs pEGFP-Rab11wt, pEGFP-Rab11Q70L and pEGFP-Rab11S25N. The stably transfected cells were mounted on coverslips and immediately analyzed by fluorescence microscopy without fixation using an inverted microscope (Nikon Eclipse TE 300, Germany) equipped with a filter system (excitation filter 450-490, barrier filter 515). Images were obtained with a CCD camera (Orca I, Hamamatsu) and processed using MetaMorph 4.5 (Universal Images Corporation).