Fig. 2. Crh2p-GFP distribution in a cdc42-1^ts strain. cdc42-1^ts cells containing the pJV40G construct were incubated either at permissive growth conditions (24°C) (A) or under restrictive growth conditions (37°C for 5 hours) (B). Polarised distribution of Crh2p was lacking at the restrictive temperature. cdc42-1^ts cells transformed with the pJV40G (Crh2-GFP) and YCp(CDC42Sc) showed a normal polarised localisation pattern at the restrictive temperature (C). Lysed cells were stained with propidium iodide.