Fig. 3. Localisation of Crh2p-GFP at the mother-bud neck depends on septin integrity. Crh2p-GFP was followed by confocal microscopy in cdc10-11 cells (VCY1) growing at permissive conditions (24°C) (A). White arrowheads indicate the typical ring-like distribution of the protein in small and medium budden cells. Cellular localisation of Crh2p-GFP in the cdc10-11 (C-E) and the isogenic wild-type cells (strain 1784) (B) growing at the restrictive temperature (37°C). Yellow arrowheads indicate the signal of fluorescence detected in the enlarged mother-bud neck region in different stages of the bud development. Two different slides of the same cells are shown in D.