Fig. 5. Localization of 5-integrin induced by FN trimer and pentamer. Cells were treated for 5 minutes with different FN-TN constructs and then fixed and labeled simultaneously with avidin to localize the FN-TN construct, and with an antibody against 5-integrin. The monomer and dimer show mostly a diffuse pattern of both FN and integrin. The trimer and pentamer show the strong localization of FN into streaks, as already described. These streaks also stained for 5 integrin, although a substantial pool of diffuse integrin remained in each case. Some plasma FN (pFN) localized over the integrin streaks, but there were also prominent FN patches localized elsewhere.