Fig. 6. Localization of FN oligomers over actin fibers. Cells were treated for 5 minutes with different FN-TN constructs and then fixed and labeled simultaneously with avidin to localize the FN-TN, and with phalloidin to localize actin polymers. The FN-TN streaks produced by the trimer and pentamer are always localized over actin fibers, but only a sub-set of the actin fibers is decorated. The streaks of plasma FN were shorter and more peripheral than those of FN-TN trimer and pentamer, and localized over shorter segments of actin fibers.