Fig. 2. PD98059 and UO126 inhibit EGF-induced desmosome disruption and promotes desmosome reformation in stable RasV12 transfectants. (A) Control NBT-II cells were cultured without (a) or with 30 ng/ml EGF (b) for 15 hours.

Alternatively, 2 days after seeding, cells were incubated with 20 µM of LY80052 (c), 25 µM of FTase2 (d), 100 µM of PD098059 (e) or 100 µM of UO126 (f) for 24 hours before an additional 15 hours incubation with 30 ng/ml of EGF. Cells were fixed and stained for DP expression. Stable RasV12-overexpressing cells (Ras32) (g) were incubated with 20 µM of LY80058 (h), 100 µM of PD098059 (i) or 100 µM of UO126 (j) for 24 hours and 25 µM of FTase2 (k) for 48 hours. (B) Phase contrast micrographs of NBT-II cells (left) and Ras32 cells (right). Note that RasV12 cells exhibit a fibroblastoid phenotype in sharp contrast to the epithelial morphology of the parental NBT-II cells. Bar, 30 µm.