Fig. 5. MCD disrupts lipid raft formation. MonoMac-6 cells were either not treated (A) or treated (B) with 10 mM MCD for 10 minutes, before solubilisation in 1% Triton X-100 buffer, followed by raft and non-raft separation by sucrose density gradient centrifugation. The GM-1 ganglioside distribution was visualised using HRP-conjugated cholera toxin.