Fig. 8. LPS signalling in lipid rafts. MonoMac-6 cells were either stimulated (A,C,E) or not stimulated with LPS (B,D,F) prior to treatment with 1% Triton X-100 buffer for 1 hour on ice and then subjected to sucrose density gradient centrifugation. Fractions were collected from the top of the gradient, 1% n-octylglucoside was added to each fraction, and equivalent portions of each fraction were analysed by SDS-PAGE and immunoblotting. The nitrocellulose membranes were probed with MyD88 (A,B), Rac-1 (C,D) or SAPK/JNK (E,F) phospho-specific antibodies followed by incubation of HRP-conjugated secondary antibodies. The relative positions of the raft and non-raft (soluble) fractions are indicated.