Fig. 2. Western blotting showed that the amount of K1 was slightly increased in K10^-/- skin (A, lane 4) compared with that in wild-type skin (A, lane 3; lanes1,2, corresponding Coomassie-stained blot). K5 (B, lane 4, K10^-/-; lane 3, wild-type; lanes 1,2, corresponding blot) and K14 (C, lane 4, K10^-/-; lane 3, wild-type; lanes 1,2, corresponding blot) were also slightly increased, whereas K15 was unchanged (D, lane 4, K10^-/-; lane 3, wild-type; lanes 1,2, corresponding blot). Immunofluorescence analysis of ear epidermis showed unaltered suprabasal K1 localization (E, wild-type; F, K10^-/-), K5 and K14 were detected in basal and suprabasal layers (K10^-/-, H and K, respectively; wild-type, G and I). K15 remained restricted to the basal layer (K10^-/-, M; and wild-type, L). Bars, 50 µm.