Fig. 5. Immunohistochemical analysis showed an increase in cyclin D1 (B) and c-Myc (D) in K10^-/- epidermis compared with the wild-type, where both proteins are exclusively found in a restricted number of basal cells (A and C, respectively). The expression of ß-catenin remained restricted to keratinocyte cell membranes (E, wild-type; F, K10^-/-). (G) Northern blotting revealed that the expression of Wnt-3 (lane 3, wt; lane 4, K10^-/-; loading control: lane 1, wt; lane 2, K10^-/-), Wnt-4 (lane 7, wt; lane 8, K10^-/-; loading control: lane 5, wt; lane 6, K10^-/-1) and Wnt-5a (lane 11, wt; lane 12, K10^-/-; loading control: lane 9, wt; lane 10, K10^-/-) were not altered in K10^-/- skin. Arrowheads point to distinct RNA species. Bar, 40 µm.